Antioxidant Activity of Roselle Flower Extract (Hibiscus sabdariffa L.) at Different Storage Durations Using the DPPH Method
DOI:
https://doi.org/10.69503/medika.v6i2.1715Keywords:
Antioxidants, DPPH, IC₅₀, Rosella, Vitamin CAbstract
Free radicals can cause cellular damage that contributes to various degenerative diseases, making antioxidant compounds necessary to neutralize them. One plant with potential as a natural source of antioxidants is the rosella flower, which is known to contain bioactive compounds such as flavonoids, phenolics, and anthocyanins. This study aims to determine the antioxidant activity of rosella flower petal extract using the DPPH method based on the IC₅₀ value and to compare it with vitamin C as a positive control. This study is a laboratory experiment using a quantitative approach. The sample used was rosella flower petals extracted via maceration using 96% ethanol as the solvent. The antioxidant activity of the extract was tested using the DPPH method, with the IC₅₀ value determined via UV-Vis spectrophotometry. The results of the study showed that the IC₅₀ value for vitamin C was 96.64 µg/mL, while those for rosella flower petal extract stored for 3 days and 5 days were 106.47 µg/mL and 104.12 µg/mL, respectively. The 5-day storage period showed better antioxidant activity compared to the 3-day period, although it was still lower than that of vitamin C. Thus, the duration of storage affects the antioxidant activity of rosella flower petal extract.
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